Heme insertion, assembly, and activation of apo-neuronal nitric-oxide synthase in vitro.

@article{Bender2000HemeIA,
  title={Heme insertion, assembly, and activation of apo-neuronal nitric-oxide synthase in vitro.},
  author={Andrew T Bender and Mikiya Nakatsuka and Yoichi Osawa},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 34},
  pages={26018-23}
}
It has been established that in the case of inducible NO synthase (NOS), a functionally active homodimer is assembled from the heme-deficient monomeric apo-NOS in vitro by the addition of heme, whereas the heme-deficient neuronal isoform (apo-nNOS) is at best only partially activated. In the current study we have discovered that reactive oxygen species, which can be removed by the addition of superoxide dismutase and catalase, destroy the heme and limit the activation of apo-nNOS in vitro. With… CONTINUE READING

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