Heme biosynthesis in Rhizobium. Identification of a cloned gene coding for delta-aminolevulinic acid synthetase from Rhizobium meliloti.

@article{Leong1982HemeBI,
  title={Heme biosynthesis in Rhizobium. Identification of a cloned gene coding for delta-aminolevulinic acid synthetase from Rhizobium meliloti.},
  author={Sally Ann Leong and Gary S. Ditta and D. R. Helinski},
  journal={The Journal of biological chemistry},
  year={1982},
  volume={257 15},
  pages={8724-30}
}
A symbiotically important gene system in rhizobial species is the heme biosynthetic pathway. A mutant having reduced levels of delta-aminolevulinic acid synthetase, the first unique enzyme in this pathway, was obtained in Rhizobium meliloti 102F34 by N-methyl-N'-nitro-N-nitrosoguanidine mutagenesis. Enzyme activity ranged from 3-13% of the wild type. Alfalfa plants inoculated with the Rhizobium synthetase mutant grew no better than uninoculated controls and formed only small white nodules which… CONTINUE READING

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