Heme binding to murine erythroleukemia cells. Evidence for a heme receptor.

@article{Galbraith1985HemeBT,
  title={Heme binding to murine erythroleukemia cells. Evidence for a heme receptor.},
  author={Richard A. Galbraith and Shigeru Sassa and A. Kappas},
  journal={The Journal of biological chemistry},
  year={1985},
  volume={260 22},
  pages={12198-202}
}
Friend virus transformed murine erythroleukemia (MEL) cells are known to take up heme from the surrounding medium and to incorporate it into newly synthesized hemoglobin (Granick, J. L., and Sassa, S. (1978) J. Biol. Chem. 253, 5402-5406), but the mechanism of its uptake is unknown. We hypothesized the existence of a specific receptor for heme in the plasma membrane. Using [55Fe]heme, we examined the characteristics of its interaction with MEL cells at 4 degrees C. [55Fe]heme binding reached… CONTINUE READING