Heme-associated subunit complex of cytochrome c oxidase identified by a new two-dimensional gel electrophoresis.

@article{Freedman1979HemeassociatedSC,
  title={Heme-associated subunit complex of cytochrome c oxidase identified by a new two-dimensional gel electrophoresis.},
  author={Jo Ann Freedman and Russell P Tracy and Siu Hin Chan},
  journal={The Journal of biological chemistry},
  year={1979},
  volume={254 11},
  pages={4305-8}
}
A Novel two-dimensional system (isoelectric focusing in the first dimension and sodium dodecyl sulfate-urea electrophoresis in the second) was used to determine the pI values of beef heart subunits of cytochrome c oxidase. The results identified a heme-associated complex of Subunits I, II, and IV. Conditions mitigate against migration of free heme.