Hematopoietic stem cell gene transfer in a tumor-prone mouse model uncovers low genotoxicity of lentiviral vector integration

@article{Montini2006HematopoieticSC,
  title={Hematopoietic stem cell gene transfer in a tumor-prone mouse model uncovers low genotoxicity of lentiviral vector integration},
  author={Eugenio Montini and Daniela Cesana and Manfred Schmidt and Francesca Sanvito and Maurilio Ponzoni and Cynthia C. Bartholomae and Lucia Sergi Sergi and Fabrizio Benedicenti and Alessandro Ambrosi and Clelia Di Serio and Claudio Doglioni and Christof von Kalle and Luigi Naldini},
  journal={Nature Biotechnology},
  year={2006},
  volume={24},
  pages={687-696}
}
Insertional mutagenesis represents a major hurdle to gene therapy and necessitates sensitive preclinical genotoxicity assays. Cdkn2a−/− mice are susceptible to a broad range of cancer-triggering genetic lesions. We exploited hematopoietic stem cells from these tumor-prone mice to assess the oncogenicity of prototypical retroviral and lentiviral vectors. We transduced hematopoietic stem cells in matched clinically relevant conditions, and compared integration site selection and tumor development… 
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Genotoxicity assay for gene therapy vectors in tumor prone Cdkn2a⁻/⁻ mice.
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