Hematopoietic stem cell expansion caused by a synthetic fragment of leptin.

Abstract

Leptin is a cytokine that regulates food intake, energy expenditure and hematopoiesis. Based on the tridimensional structure of the human leptin molecule, six fragments have been synthesized, (Ac-Lep23-47-NH2, [LEP1]; Ac-Lep48-71-NH2, [LEP2]; Ac-Lep72-88-NH2, [LEP3]; Ac-Lep92-115-NH2, [LEP4], Ac-[Ser(117)]-Lep116-140-NH2, [LEP5] and Ac-Lep141-164-NH2, [LEP6]), and their effects on hematopoiesis were evaluated. The mice were treated with 1mg/kg LEP5 for 3 days. The mature and primitive hematopoietic populations were quantified. We observed that the mature populations from the bone marrow and spleen were not affected by LEP5. However, the peptide caused at least a two-fold increase in the number of hematopoietic stem cells, the most primitive population of the bone marrow. Additionally, the number of granulocyte/macrophage colony-forming units produced by bone marrow cells in methylcellulose also increased by 40% after treatment with LEP5, and the leptin receptor was activated. These results show that the leptin fragment LEP5 is a positive modulator of the in vivo expansion of hematopoietic stem cells.

DOI: 10.1016/j.peptides.2013.09.012

2 Figures and Tables

0102030201520162017
Citations per Year

Citation Velocity: 8

Averaging 8 citations per year over the last 3 years.

Learn more about how we calculate this metric in our FAQ.

Cite this paper

@article{Dias2013HematopoieticSC, title={Hematopoietic stem cell expansion caused by a synthetic fragment of leptin.}, author={Carol C. Dias and Amanda Nogueira-Pedro and Christiano M.V. Barbosa and Antonio Carlos Ribeiro-Filho and Frederick Wasinski and Ronaldo de Carvalho Ara{\'u}jo and Vani Xavier de Oliveira and Antonio Miranda and Edgar Julian Paredes-Gamero}, journal={Peptides}, year={2013}, volume={50}, pages={24-7} }