Hematopoietic colony-forming cells derived from human embryonic stem cells.

  title={Hematopoietic colony-forming cells derived from human embryonic stem cells.},
  author={Dan S Kaufman and Eric T. Hanson and Rachel L. Lewis and Robert D. Auerbach and James A. Thomson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={98 19},
Human embryonic stem (ES) cells are undifferentiated, pluripotent cells that can be maintained indefinitely in culture. Here we demonstrate that human ES cells differentiate to hematopoietic precursor cells when cocultured with the murine bone marrow cell line S17 or the yolk sac endothelial cell line C166. This hematopoietic differentiation requires fetal bovine serum, but no other exogenous cytokines. ES cell-derived hematopoietic precursor cells express the cell surface antigen CD34 and the… CONTINUE READING
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In Vitro Cell

  • S. J. Wang, P. Greer, R. Auerbach
  • Dev . Biol . Anim .
  • 1996

Atlas of Human Hematopoietic Colonies (StemCell Technologies, Vancouver)

  • C. Eaves, K. Lambie
  • 1995

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