Hematin polymerization assay as a high-throughput screen for identification of new antimalarial pharmacophores.

@article{Kurosawa2000HematinPA,
  title={Hematin polymerization assay as a high-throughput screen for identification of new antimalarial pharmacophores.},
  author={Yae Kurosawa and Arnulf Dorn and M Kitsuji-Shirane and Shinichi Kyotanabe-shi Tanaka and Takatomo Satoh and Hugues Matile and Werner Hofheinz and Raffaello Masciadri and Manfred Kansy and Robert G. Ridley},
  journal={Antimicrobial agents and chemotherapy},
  year={2000},
  volume={44 10},
  pages={
          2638-44
        }
}
Hematin polymerization is a parasite-specific process that enables the detoxification of heme following its release in the lysosomal digestive vacuole during hemoglobin degradation, and represents both an essential and a unique pharmacological drug target. We have developed a high-throughput in vitro microassay of hematin polymerization based on the detection of (14)C-labeled hematin incorporated into polymeric hemozoin (malaria pigment). The assay uses 96-well filtration microplates and… CONTINUE READING
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