Hematin as a peroxidase substitute in hydrogen peroxide determinations.

  title={Hematin as a peroxidase substitute in hydrogen peroxide determinations.},
  author={Zhang Genfa and Purnendu K. Dasgupta},
  journal={Analytical chemistry},
  volume={64 5},
Hematin can substitute for horseradish peroxidase (HRP) as the catalyst in the determination of hydrogen peroxide using phenolic substrates such as p-hydroxyphenylacetate or p-cresol. Although the peroxidatic activity of hematin from bovine blood is not as great as HRP in terms of unit iron content, the activity per unit weight is substantially greater. Hematin is 500 times less expensive than HRP per unit peroxidatic activity. In hematin-catalyzed systems, reaction development and fluorescence… CONTINUE READING


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