Hemangioblastic characteristics of fetal bone marrow-derived Flk1(+)CD31(-)CD34(-) cells.

@article{Guo2003HemangioblasticCO,
  title={Hemangioblastic characteristics of fetal bone marrow-derived Flk1(+)CD31(-)CD34(-) cells.},
  author={Hong Guo and Baijun Fang and Lianming Liao and Zhigang Zhao and Jiewen Liu and Huishu Chen and Steven H Hsu and Qi Qi Cui and Robort Chunhua Zhao},
  journal={Experimental hematology},
  year={2003},
  volume={31 7},
  pages={650-8}
}
OBJECTIVE To investigate whether Flk1(+)CD31(-)CD34(-) cells isolated from fetal bone marrow (BM) have characteristics of hemangioblasts, i.e., progenitors of endothelial and hematopoietic cells. MATERIALS AND METHODS Mononuclear cells from fetal BM were negatively sorted by CD45, GlyA, and CD34 micromagnetic beads, then cultured to form cell colonies. A single colony was harvested. Culture-expanded cells were seeded on ECM gel or semisolid media supplemented with endothelial and… CONTINUE READING