Helminth endoparasites of the smooth newt Lissotriton vulgaris: linking morphological identification and molecular data

  title={Helminth endoparasites of the smooth newt Lissotriton vulgaris: linking morphological identification and molecular data},
  author={Ulrich Sinsch and Petr Heneberg and Miroslav Tě{\vs}{\'i}nsk{\'y} and Carsten Balczun and Patrick L. Scheid},
  journal={Journal of Helminthology},
  pages={332 - 341}
Abstract The helminth endoparasites of many European amphibian species are often known exclusively from morphological descriptions. A molecular library of DNA sequence data linked to morphological identifications is still in its infancy. In this paper, we aim to contribute to such a library on the smooth newt Lissotriton vulgaris, the intermediate and definitive host of 31 helminth parasites, according to evidence published so far. Newts (n = 69) were collected at two study sites in western… 
Molecular phylogeny of Megalobatrachonema (Nematoda: Ascaridida), with description of a new species based on morphological and molecular evidence.
Molecular Diversity of Nematode Parasites in Afrotropical Reed Frogs (Hyperolius spp.)
The findings suggest that nematode diversity is probably lower than previously assumed due to low host specificity, and data facilitate molecular identification of adult and specifically larval nematodes found in amphibians of Sub-Saharan Africa.
Description of a new species of Aplectana (Nematoda: Ascaridomorpha: Cosmocercidae) using an integrative approach and preliminary phylogenetic study of Cosmocercidae and related taxa
A basic molecular phylogenetic framework for the superfamily Cosmocercoidea based on 18S + 28S sequence data is provided for the first time to the authors' knowledge and a new species, A. xishuangbannaensis n.
Molecular phylogeny of the family Cosmocercidae (Nematoda: Ascaridida), with description of a new species of Aplectana using an integrative approach
The basic molecular phylogenetic framework for the superfamily Cosmocercoidea based on 18S + 28S sequence data for the first time is provided and Morphological and genetic evidence supported that the nematode specimens collected from P. megacephalus represents a new species of Aplectana.
Oswaldocruzia filiformis sensu lato (Nematoda: Molineidae) from amphibians and reptiles in European Russia: morphological and molecular data
Molecular data indicate that only one species of the genus Oswaldocruzia, O. filiformis s.l., which has a broad morphological variability, parasitises amphibians and reptiles in European Russia.
WHAT'S NEW IN THE SCIENTIFIC LITERATURE Infectious Diseases of Reptiles and Amphibians: Peer-reviewed publications, January-July 2019
The first reptilian circovirus identified infects gut and liver tissues of black-headed pythons, and the first novel herpesvirus associated with oropharyngeal squamous cell carcinoma in smooth green snakes is identified.
The fast–slow continuum of longevity among yellow-bellied toad populations (Bombina variegata): intrinsic and extrinsic drivers of variation
It is proposed that a population’s position within the fast–slow continuum integrates the response to local environmental stochasticity and the efficiency of chemical antipredator protection determining the magnitude of longevity at the local and the continental scale.


Metacercariae of Clinostomum complanatum (Trematoda: Digenea) in European newts Triturus carnifex and Lissotriton vulgaris (Caudata: Salamandridae)
This is the first record of C. complanatum in European newts and, more generally, in amphibians in Europe, and sequences of a mitochondrial gene, cytochrome c oxidase I, and the ribosomal markers, internal transcribed spacers are identified.
Linking Larvae and Adults of Apharyngostrigea cornu, Hysteromorpha triloba, and Alaria mustelae (Diplostomoidea: Digenea) Using Molecular Data
abstract:  Because the taxonomy of trematodes is based on adults, the larval stages of most digeneans cannot be identified to species based on morphology alone. Molecular data provide a means of
Morphological Characteristics and Phylogenetic Trends of Trematode Cercariae in Freshwater Snails from Nakhon Nayok Province, Thailand
This investigation confirmed that the ITS2 data of cercariae could be used to study on phylogenetic relationships or to determine classification of this species at order and/or family level when possible.
Central European parasitic flatworms of the Cyclocoelidae Stossich, 1902 (Trematoda: Plagiorchiida): molecular and comparative morphological analysis suggests the reclassification of Cyclocoelum obscurum (Leidy, 1887) into the Harrahium Witenberg, 1926
Cyclocoelids found during the extensive examination of central European birds performed from 1962 to 2016 are found, comparative measurements, host spectra, prevalence and intensity, and sequences of four DNA loci of five of the cyclocoelid species are provided.
The ribosomal RNA gene sequences of the metacercariae collected from salamanders and the E. costaricensis collected from a Japanese marten were identical, confirming the morphology-based species identification.
Nematode Species Identification—Current Status, Challenges and Future Perspectives for Cyathostomins
Recent advances in proteomic tools for identification of metazoans including insects and nematodes of the genus Trichinella will be evaluated for suitability to diagnose cyathostomins.
Selective and universal primers for trematode barcoding in freshwater snails
The specific oligonucleotide primer was universal for trematodes that infected several freshwater snail species in Israel and created a species barcoding system that classifies species accurately due to high polymorphism between conserved areas.
Helminth Parasites of the Caucasian Salamander, Mertensiella caucasica, from Turkey
Seventy-two Caucasian salamanders collected from 5 localities in Turkey between 1995 and 2001 and examined for helminths were examined and one new host record and 6 new locality records are reported.