Helleborus net necrosis virus: A New Carlavirus Associated with 'Black Death' of Helleborus spp.

  title={Helleborus net necrosis virus: A New Carlavirus Associated with 'Black Death' of Helleborus spp.},
  author={K. Eastwell and L. D. du Toit and K. Druffel},
  journal={Plant disease},
  volume={93 4},
  • K. Eastwell, L. D. du Toit, K. Druffel
  • Published 2009
  • Biology, Medicine
  • Plant disease
  • Symptoms of 'black death' were observed on Helleborus spp. in each of three independent nurseries from across the United States. A new virus of the genus Carlavirus was identified in association with this disease. Symptomatic plants contained curved, rod-shaped particles averaging 800 by 17 nm, and yielded predominant bands of double-stranded (ds)RNA corresponding to approximately 9.0, 2.6, and 1.7 kbp. Amplification with degenerate primers for carlaviruses yielded a product of approximately 3… CONTINUE READING
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