Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution

@inproceedings{Joens2013HeliumIM,
  title={Helium Ion Microscopy (HIM) for the imaging of biological samples at sub-nanometer resolution},
  author={Matthew S. Joens and Chuong Huynh and James M. Kasuboski and David Ferranti and Yury J. Sigal and Fabian Zeitvogel and Martin Obst and Claus J. Burkhardt and Kevin P. Curran and Sreekanth H. Chalasani and Lewis Stern and Bernhard Goetze and James A. J. Fitzpatrick},
  booktitle={Scientific reports},
  year={2013}
}
Scanning Electron Microscopy (SEM) has long been the standard in imaging the sub-micrometer surface ultrastructure of both hard and soft materials. In the case of biological samples, it has provided great insights into their physical architecture. However, three of the fundamental challenges in the SEM imaging of soft materials are that of limited imaging resolution at high magnification, charging caused by the insulating properties of most biological samples and the loss of subtle surface… CONTINUE READING