Helicase SPRNTing through the nanopore.

Enzymes that move directionally on single-stranded nucleic acids are at the core of emerging nanopore sequencing technology. Of a particular use are DNA helicases, molecular motors that bind single-stranded DNA (ssDNA) independently of its sequence and use ATP to fuel their directional motion along the DNA (1). In nanopore-based sequencing, a pore formed by… CONTINUE READING