Heightened self-reactivity associated with selective survival, but not expansion, of naïve virus-specific CD8+ T cells in aged mice.

Abstract

In advanced age, decreased CD8(+) cytotoxic T-lymphocyte (CTL) responses to novel pathogens and cancer is paralleled by a decline in the number and function of naïve CTL precursors (CTLp). Although the age-related fall in CD8(+) T-cell numbers is well established, neither the underlying mechanisms nor the extent of variation for different epitope specificities have been defined. Furthermore, naïve CD8(+) T cells expressing high levels of CD44 accumulate with age, but it is unknown whether this accumulation reflects their preferential survival or an age-dependent driver of CD8(+) T-cell proliferation. Here, we track the number and phenotype of four influenza A virus (IAV)-specific CTLp populations in naïve C57BL/6 (B6) mice during aging, and compare T-cell receptor (TCR) clonal diversity for the CD44hi and CD44lo subsets of one such population. We show differential onset of decline for several IAV-specific CD8(+) T-cell populations with advanced age that parallel age-associated changes in the B6 immunodominance hierarchy, suggestive of distinct impacts of aging on different epitope-specific populations. Despite finding no evidence of clonal expansions in an aged, epitope-specific TCR repertoire, nonrandom alterations in TCR usage were observed, along with elevated CD5 and CD8 coreceptor expression. Collectively, these data demonstrate that naïve CD8(+) T cells expressing markers of heightened self-recognition are selectively retained, but not clonally expanded, during aging.

DOI: 10.1073/pnas.1525167113

Cite this paper

@article{Quinn2016HeightenedSA, title={Heightened self-reactivity associated with selective survival, but not expansion, of na{\"{i}ve virus-specific CD8+ T cells in aged mice.}, author={Kylie M. Quinn and Sophie G. Zaloumis and Tania Cukalac and Wan-Ting Kan and Xavier Y X Sng and Michiko Mirams and Katherine A. Watson and James M. McCaw and Peter Doherty and Paul G Thomas and Andreas Handel and Nicole L La Gruta}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={2016}, volume={113 5}, pages={1333-8} }