Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging.

@article{Ong2015HeavyWA,
  title={Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging.},
  author={Wei Qiang Ong and Yael Citron and Joerg Schnitzbauer and Daichi Kamiyama and Bo Huang},
  journal={Chemical communications},
  year={2015},
  volume={51 70},
  pages={13451-3}
}
Photoactivatable fluorescent proteins (PA-FPs) are widely used in live single-molecule super-resolution imaging but emit substantially fewer photons than organic dyes do. Herein, we show that in heavy water (D2O) instead of H2O, common PA-FPs emit 26-54% more photons, effectively improving the localization precision in super-resolution imaging. 
Recent Discussions
This paper has been referenced on Twitter 8 times over the past 90 days. VIEW TWEETS