Heavy Metal-Induced Differential Gene Expression of Metallothionein in Javanese Medaka, Oryzias javanicus

@article{Woo2006HeavyMD,
  title={Heavy Metal-Induced Differential Gene Expression of Metallothionein in Javanese Medaka, Oryzias javanicus},
  author={Seonock Woo and Seungshic Yum and Jee Hyun Jung and Won Joon Shim and Chang-Hoon Lee and Taek-Kyun Lee},
  journal={Marine Biotechnology},
  year={2006},
  volume={8},
  pages={654-662}
}
A metallothionein (MT) gene was isolated for the first time from Javanese medaka, Oryzias javanicus, which shows high adaptability from freshwater to seawater. The full-length cDNA of MT from O. javanicus (OjaMT) comprises 349 bp, excluding the poly(A)+ stretch, and codes for a total of 60 amino acids. The positions of cysteine residues are highly conserved. The pattern of OjaMT expression induced by six heavy metals was analyzed via real-time quantitative polymerase chain reaction (PCR). The… CONTINUE READING