Heat-stable enterotoxin activation of immunopurified guanylyl cyclase C. Modulation by adenine nucleotides.

@article{Vaandrager1993HeatstableEA,
  title={Heat-stable enterotoxin activation of immunopurified guanylyl cyclase C. Modulation by adenine nucleotides.},
  author={Arie B. Vaandrager and Els C W van der Wiel and Hugo R. de Jonge},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 26},
  pages={19598-603}
}
We studied the activation and inactivation of recombinant guanylyl cyclase (GC) C stably expressed in human kidney 293 cells. Activation of GC-C by heat-stable enterotoxin (STa), Cd2+, hemin, or the detergent Triton X-100 was followed by a rapid inactivation of the enzyme. Adenine nucleotides were found to prevent the inactivation process in native membranes, as well as following enzyme solubilization and immunopurification. Inactivation of GC-C was not associated with dephosphorylation… CONTINUE READING

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