Heat-shock response down-regulates interleukin-18 expression in murine peritoneal macrophages.


BACKGROUND INFORMATION The heat-shock response is a self-defence mechanism that protects cells and organisms from a wide range of harmful stresses. Recent studies revealed that it involved the regulation of cytokine expression. Interleukin-18 (IL-18) is an important cytokine in mediating immune response. RESULTS We studied interferon-gamma (IFN-gamma)-induced IL-18 expression in heat-shock-treated murine peritoneal macrophages. Our results showed that the heat-shock response significantly inhibited the expression of IFN-gamma-induced pro-inflammatory cytokine IL-18. Interferon consensus sequence binding protein (ICSBP) is a transcription factor that binds to the promoter of IL-18 and regulates the transcription of IL-18. Further research on the down-regulation mechanism showed that the DNA-binding activity of ICSBP was greatly reduced by the heat shock response. CONCLUSIONS These results suggest that the inhibitory effect of heat-shock response on IL-18 production in IFN-gamma-stimulated macrophages is related to the suppression of the binding activity of ICSBP.

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@article{Wang2005HeatshockRD, title={Heat-shock response down-regulates interleukin-18 expression in murine peritoneal macrophages.}, author={Yun Wang and Zongliang Chang}, journal={Biology of the cell}, year={2005}, volume={97 7}, pages={551-6} }