Heat-shock induction of RNA polymerase sigma-32 synthesis in Escherichia coli: Transcriptional control and a multiple promoter system

@article{Fujita1987HeatshockIO,
  title={Heat-shock induction of RNA polymerase sigma-32 synthesis in Escherichia coli: Transcriptional control and a multiple promoter system},
  author={Nobuyuki Fujita and Akira Ishihama},
  journal={Molecular and General Genetics MGG},
  year={1987},
  volume={210},
  pages={10-15}
}
Transcriptional start sites of the rpoH gene which codes for a minor σ factor (σ32) of Escherichia coli RNA polymerase were determined. The rpoH gene is transcribed, both in vivo and in vitro, from two major (P1 and P2) and one minor (P2*) promoters. In vitro synthesis of the rpoH mRNAs is dependent on the major species of RNA polymerase holoenzyme (Eσ70) but not on the minor one (Eσ32). S1 nuclease analysis of the in vivo RNA showed that the level of rpoH transcript from the downstream P2… CONTINUE READING
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