Heat shock induced excision of selectable marker genes in transgenic banana by the Cre-lox site-specific recombination system.

@article{ChongPrez2010HeatSI,
  title={Heat shock induced excision of selectable marker genes in transgenic banana by the Cre-lox site-specific recombination system.},
  author={Borys Chong-P{\'e}rez and Rafael G{\'o}mez Kosky and Maritza Reyes and Luis Roberto Llerena Rojas and B{\'a}rbara Maribel River{\'o}n Oca{\~n}a and Marisol Tejeda and Blanca P{\'e}rez and Geert Angenon},
  journal={Journal of biotechnology},
  year={2010},
  volume={159 4},
  pages={
          265-73
        }
}
Selectable marker genes are indispensable for efficient production of transgenic events, but are no longer needed after the selection process and may cause public concern and technological problems. Although several gene excision systems exist, few have been optimized for vegetatively propagated crops. Using a Cre-loxP auto-excision strategy, we obtained transgenic banana plants cv. Grande Naine (Musa AAA) devoid of the marker gene used for selection. We used T-DNA vectors with the cre… CONTINUE READING

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