Heat shock causes protein aggregation and reduced protein solubility at the centrosome and other cytoplasmic locations.

@article{Vidair1996HeatSC,
  title={Heat shock causes protein aggregation and reduced protein solubility at the centrosome and other cytoplasmic locations.},
  author={Charles Vidair and Rong Nan Huang and Stephen John Doxsey},
  journal={International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group},
  year={1996},
  volume={12 5},
  pages={681-95}
}
Heat shock markedly inhibited centrosome staining by antisera raised against the two centrosome-specific proteins, pericentrin and gamma tubulin. The inhibition of anti-pericentrin binding was measured by fluorescence imaging. Heat had the greatest effect on intact cells, followed in sensitivity by centrosomes attached to their companion nucleus, with purified centrosomes being least sensitive. The centrosomal content of pericentrin was measured by immunoprecipitation followed by western… CONTINUE READING