Heat-mediated immune complex dissociation and enzyme-linked immunosorbent assay signal amplification render p24 antigen detection in plasma as sensitive as HIV-1 RNA detection by polymerase chain reaction.

@article{Schpbach1996HeatmediatedIC,
  title={Heat-mediated immune complex dissociation and enzyme-linked immunosorbent assay signal amplification render p24 antigen detection in plasma as sensitive as HIV-1 RNA detection by polymerase chain reaction.},
  author={J{\"o}rg Sch{\"u}pbach and Markus Flepp and D Pontelli and Zuzana Tomasik and Ruedi Luethy and J{\"u}rg B{\"o}ni},
  journal={AIDS},
  year={1996},
  volume={10 10},
  pages={1085-90}
}
OBJECTIVE To compare heat denaturation and acidification as immune complex dissociation (ICD) methods in adult HIV-1 infection and to increase the sensitivity by a signal-amplification-boosted HIV-1 p24 antigen enzyme-linked immunosorbent assay (ELISA). DESIGN Prospective and retrospective blinded study of paired serum and plasma samples from 245 patients (112 of class A, 66 of B, 67 of C of the Centers for Disease Control and Prevention 1993 classification). METHODS Plasma and sera were… CONTINUE READING

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