Heat-labile uracil-DNA glycosylase: purification and characterization.

  title={Heat-labile uracil-DNA glycosylase: purification and characterization.},
  author={H. Sobek and Marion Schmidt and Brendan Frey and Klaus Kaluza},
  journal={FEBS letters},
  volume={388 1},
A uracil-DNA glycosylase (UNG) from a psychrophilic marine bacterium (BMTU 3346) has been purified to apparent homogeneity. The enzyme has a molecular weight of 23400 Da. It is stable in complex buffers (containing glycerol/BSA), whereas it is heat-labile in dilute buffers (free of stabilizers) with a half-life of 2 min at 40 degrees C. Due to the thermolability, uracil-DNA glycosylase is suitable for application in the carryover prevention technique showing less residual activity and/or a… CONTINUE READING

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