Heat-inducible transgenic expression in the silkmoth Bombyx mori

  title={Heat-inducible transgenic expression in the silkmoth Bombyx mori},
  author={Mirka Uhl{\'i}řov{\'a} and Masako Asahina and Lynn M Riddiford and Marek Jindra},
  journal={Development Genes and Evolution},
Germline transformation with new transposon vectors now enables causal tests of gene function via ectopic protein expression or RNA interference in non-drosophilid insects. The problem remains of how to drive the transgene expression in vivo. We employed germline transformation using the piggyBac 3xP3-EGFP vector to test whether the Drosophila heat shock hsp70 promoter will be active in the live silkworm. We modified the original vector by cloning the coding sequence for Bombyx nuclear receptor… CONTINUE READING

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