Heat-inducible autolytic vector for high-throughput screening.

@article{Xu2006HeatinducibleAV,
  title={Heat-inducible autolytic vector for high-throughput screening.},
  author={Lihua Xu and Shuang Li and Chuan Ren and Zhen Cai and Zhanglin Lin},
  journal={BioTechniques},
  year={2006},
  volume={41 3},
  pages={
          319-23
        }
}
In directed evolution, a high-throughput screening system is often a prerequisite for sampling the enzyme variants. When the target enzyme is expressed intracellularly, for example when Escherichia coli is used as the host, chemical or enzymatic disruption of cell membrane is often required in many cases, which can be tedious, time-consuming, and costly. In this study, a set of heat-inducible autolytic vectors were constructed to solve this problem, in which the SRRz lysis gene cassette from… CONTINUE READING