Heat activation/shock temperatures for Bacillus anthracis spores and the issue of spore plate counts versus true numbers of spores.

@article{Turnbull2007HeatAT,
  title={Heat activation/shock temperatures for Bacillus anthracis spores and the issue of spore plate counts versus true numbers of spores.},
  author={Peter C. B. Turnbull and Dody A Frawley and Robert L Bull},
  journal={Journal of microbiological methods},
  year={2007},
  volume={68 2},
  pages={353-7}
}
Assessing true numbers of viable anthrax spores is complex. Optimal heat activation conditions vary with species, media and germinants. Published time/temperature combinations for Bacillus anthracis spores range from 60 degrees C for <or=90 min to boiling for 1 min. Results presented here indicate that temperatures are best kept to <or=70 degrees C and holding times need not exceed 15-30 min. Under conditions of 60 degrees C for 90 min, 62-23 degrees C for 15 min and 70 degrees C for 15 or 30… CONTINUE READING

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