Heat-activatable primers for hot-start PCR: oligonucleotide synthesis and basic PCR setup.

@article{Lebedev2009HeatactivatablePF,
  title={Heat-activatable primers for hot-start PCR: oligonucleotide synthesis and basic PCR setup.},
  author={Alexandre V. Lebedev},
  journal={Current protocols in nucleic acid chemistry},
  year={2009},
  volume={Chapter 4},
  pages={
          Unit 4.35 1-17
        }
}
2'-Deoxyribonucleoside-3'-O-(4-oxotetradec-1-yl) phosphoramidites (OXT phosphoramidites) are used to prepare modified oligodeoxyribonucleotide primers containing heat cleavable OXT phosphotriester protecting groups at 3'-ultimate and penultimate internucleotide linkages. The OXT-modified primers significantly improve performance of the polymerase chain reaction (PCR) compared to standard DNA primers by substantially reducing or eliminating the accumulation of PCR artifacts such as dimerized… CONTINUE READING
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