Heat Shock Affects Permeability and Resistance of Bacillus stearothermophilus Sporest

Abstract

Heat shock of dormant spores of Bacillus stearothermophilus ATCC 7953 at 100 or 80°C for short times, the so-called activation or breaking of dormancy, was investigated by separating the resulting spores by buoyant density centrifugation into a band at 1.240 g/ml that was distinct from another band at 1.340 g/ml, the same density as the original spores. The proportion of spores at 1.240 g/ml became larger when the original dormant spores were heated for a longer period of time, but integument-stripped dormant spores were quickly and completely converted to spores with a band at 1.240 g/ml. The spores with bands at both 1.240 and 1.340 g/ml were germinable faster than the original dormant spores and thus were considered to be activated. The spores with a band at 1.240 g/ml, which were considered to be fully activated, were apparently permeabilized, with

4 Figures and Tables

Cite this paper

@inproceedings{BeamanHeatSA, title={Heat Shock Affects Permeability and Resistance of Bacillus stearothermophilus Sporest}, author={T C Beaman and H. Stuart Pankratz and Philipp Gerhardt} }