HeLa cells 50 years on: the good, the bad and the ugly
@article{Masters2002HeLaC5, title={HeLa cells 50 years on: the good, the bad and the ugly}, author={John R. W. Masters}, journal={Nature Reviews Cancer}, year={2002}, volume={2}, pages={315-319} }
HeLa cells — the first continuous cancer cell line — have been a mainstay of cancer research ever since their isolation from the aggressive glandular cervical cancer of a young woman more than 50 years ago. Knowledge of almost every process that occurs in human cells has been obtained using HeLa cells and the many other cell lines that have since been isolated. So why does fraud an ignorance surround the use of these and other human cancer cell lines?
443 Citations
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Though the HeLa cell line has contributed to many advancements in biomedical research since the twentieth century, its usage in medical research has been controversial because Lacks did not consent to having her cells used for such purposes.
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- BiologyArchives of pathology & laboratory medicine
- 2009
The history behind the development of HeLa cells, including the first published description of Ms Lacks' autopsy, and the cell culture contamination that resulted are described.
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- Biology
- 2013
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- Biology
- 2021
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References
SHOWING 1-10 OF 67 REFERENCES
Establishment of a continuous tumor‐cell line (PANC‐1) from a human carcinoma of the exocrine pancreas
- Biology, MedicineInternational journal of cancer
- 1975
An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times and has a modal number of 63 with three distinct marker chromosomes and a small ring chromosome.
HeLa cultures defined.
- BiologyScience
- 1976
A list is presented of references to all known publications on properties which have served to relate strains of HeLa cells to each other as well as to indict other purported human cell lines as HeLa…
The establishment of strains of human cells in tissue culture.
- BiologyJournal of immunology
- 1957
Five human cell lines derived from human embryonic intestine, liver, lung, skin and muscle explants, and from a liver biopsy establish epithelial-like cells and small, round, or irregularly shaped cells which are indistinguishable from McCulloch's MCN cells obtained from leukemic bone marrow.
Propagation in a Fluid Medium of a Human Epidermoid Carcinoma, Strain KB
- MedicineProceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine
- 1955
An epidermoid carcinoma of the floor of the mouth was cultivated directly onto a glass surface in a medium consisting of 13 amino acids, 7 vitamins, glucose, salts, and 10% human serum, and all were used at concentrations permitting maximal growth of that cell.
Cross-contamination of cells in culture.
- BiologyScience
- 1981
Lists are presented of references to all known publications describing cell properties that serve to characterize (i) known strains of HeLa and purported human cell lines indicated as HeLa…
The Maintenance of Human Normal Cells and Tumor Cells in Continuous Culture: I. Preliminary Report: Cultivation of Mesoblastic Tumors and Normal Tissue and Notes on Methods of Cultivation
- Biology
- 1936
The value of this report lies not in the presentation of new and unique methods for maintaining tissue cultures under continuous cultivation, but in presenting evidence that very different methods and media can be used to obtain approximately the same end-results.
Apparent HeLa Cell Contamination of Human Heteroploid Cell Lines
- BiologyNature
- 1968
A survey of twenty heteroploid human cell lines for glucose-6-phosphate de-hydrogenase (G6PD) and phosphoglucomutase (PGM) electrophoretic polymorphisms revealed that all had the same G6PD and PGM phenotypes.
Cell cross-contamination in cell cultures: The silent and neglected danger
- BiologyIn Vitro Cellular & Developmental Biology - Animal
- 1998
The problem of cell cross-contamination in cell cultures is a common problem during cell culturing and use and can be solved by increasing the awareness of its seriousness and by introducing regular quality control in every laboratory where cells are grown and used.