Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome.

@article{Riber2006HdamediatedIO,
  title={Hda-mediated inactivation of the DnaA protein and dnaA gene autoregulation act in concert to ensure homeostatic maintenance of the Escherichia coli chromosome.},
  author={Leise Riber and J. Nilsson a. N. Olsson and Rasmus Bovbjerg Jensen and Ole Skovgaard and Santanu Dasgupta and M. G. Marinus and Anders L\obner-Olesen},
  journal={Genes & development},
  year={2006},
  volume={20 15},
  pages={2121-34}
}
Initiation of DNA replication in Eschericia coli requires the ATP-bound form of the DnaA protein. The conversion of DnaA-ATP to DnaA-ADP is facilitated by a complex of DnaA, Hda (homologous to DnaA), and DNA-loaded beta-clamp proteins in a process termed RIDA (regulatory inactivation of DnaA). Hda-deficient cells initiate replication at each origin mainly once per cell cycle, and the rare reinitiation events never coincide with the end of the origin sequestration period. Therefore, RIDA is not… CONTINUE READING