Harpin induces mitogen-activated protein kinase activity during defence responses in Arabidopsis thaliana suspension cultures

@article{Desikan1999HarpinIM,
  title={Harpin induces mitogen-activated protein kinase activity during defence responses in Arabidopsis thaliana suspension cultures},
  author={Radhika Desikan and A. P. Clarke and Paul A Atherfold and John Travers Hancock and Steven John Neill},
  journal={Planta},
  year={1999},
  volume={210},
  pages={97-103}
}
Elicitation of Arabidopsis thaliana (L.) Heynh. suspension cultures with the bacterial protein harpin (from Pseudomonas syringae pv. syringae) induced the activation of two kinases of 39 and 44 kDa, as demonstrated by in-gel kinase assays using myelin basic protein (MBP) as a substrate. Both these kinases appeared to be tyrosine-phosphorylated upon activation, as demonstrated by treatment with tyrosine phosphatase and immunoprecipitation using an anti-phosphotyrosine monoclonal antibody. An… CONTINUE READING

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