Harnessing the chemical activation inherent to carrier protein-bound thioesters for the characterization of lipopeptide fatty acid tailoring enzymes.

@article{Kopp2008HarnessingTC,
  title={Harnessing the chemical activation inherent to carrier protein-bound thioesters for the characterization of lipopeptide fatty acid tailoring enzymes.},
  author={Florian Kopp and Uwe Linne and Markus Oberth{\"u}r and Mohamed A. Marahiel},
  journal={Journal of the American Chemical Society},
  year={2008},
  volume={130 8},
  pages={2656-66}
}
Here, we report a new experimental approach utilizing an amide ligation reaction for the characterization of acyl carrier protein (ACP)-bound reaction intermediates, which are otherwise difficult to analyze by traditional biochemical methods. To explore fatty acid tailoring enzymes of the calcium-dependent antibiotic (CDA) biosynthetic pathway, this strategy enabled the transformation of modified fatty acids, covalently bound as thioesters to an ACP, into amide ligation products that can be… CONTINUE READING