Harnessing recombination to speed adaptive evolution in Escherichia coli.

@article{Winkler2012HarnessingRT,
  title={Harnessing recombination to speed adaptive evolution in Escherichia coli.},
  author={James D. Winkler and Katy C Kao},
  journal={Metabolic engineering},
  year={2012},
  volume={14 5},
  pages={
          487-95
        }
}
Evolutionary engineering typically involves asexual propagation of a strain to improve a desired phenotype. However, asexual populations suffer from extensive clonal interference, a phenomenon where distinct lineages of beneficial clones compete and are often lost from the population given sufficient time. Improved adaptive mutants can likely be generated by genetic exchange between lineages, thereby reducing clonal interference. We present a system that allows continuous in situ recombination… CONTINUE READING
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