Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC

@article{Li2007HarnessingHR,
  title={Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC},
  author={Mamie Z. Li and Stephen J. Elledge},
  journal={Nature Methods},
  year={2007},
  volume={4},
  pages={251-256}
}
We describe a new cloning method, sequence and ligation–independent cloning (SLIC), which allows the assembly of multiple DNA fragments in a single reaction using in vitro homologous recombination and single-strand annealing. SLIC mimics in vivo homologous recombination by relying on exonuclease-generated ssDNA overhangs in insert and vector fragments, and the assembly of these fragments by recombination in vitro. SLIC inserts can also be prepared by incomplete PCR (iPCR) or mixed PCR. SLIC… Expand
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