Haptoglobin subtyping by isoelectric focusing in ultrathin-layer polyacrylamide gels population genetic data for Hanover and Lower Saxony

@article{Rothmel1989HaptoglobinSB,
  title={Haptoglobin subtyping by isoelectric focusing in ultrathin-layer polyacrylamide gels population genetic data for Hanover and Lower Saxony},
  author={Thomas Roth{\"a}mel and H. P. Kr{\"u}ger and Hans Dieter Tr{\"o}ger},
  journal={Zeitschrift f{\"u}r Rechtsmedizin},
  year={1989},
  volume={102},
  pages={391-397}
}
This report describes a method for subtyping haptoglobin by means of isoelectric focusing in 0.2-mm ultrathin-layer polyacrylamide gels. Haptoglobin (Hp) is purified by ion-exchange chromatography and reduced. The well-known advantages of ultrathin-layer gels combine high isoelectrophoretic resolution of the Hp subtypes with less demands for time and material and make sequential visualization by fixation and protein staining possible. The distribution of the Hp subtypes in 1500 unrelated adults… CONTINUE READING

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