Haploidy influences Bak and Bcl-xL mRNA expression and increases incidence of apoptosis in porcine embryos.

@article{Jeong2005HaploidyIB,
  title={Haploidy influences Bak and Bcl-xL mRNA expression and increases incidence of apoptosis in porcine embryos.},
  author={Yu-Jeong Jeong and Xiang-Shun Cui and Jin Young Kim and Il Hwa Kim and Teoan Kim and Yon-Bok Chung and N Iu Kim},
  journal={Zygote},
  year={2005},
  volume={13 1},
  pages={17-21}
}
The objective of this study was to determine developmental pattern, total cell number, apoptosis and apoptosis-related gene expression in haploid and diploid embryos following parthenogenetic activation. In vitro-matured porcine oocytes were activated by electrical pulses and cultured in the absence or presence of cytochalasin B for 3 h. Zygotes with two polar bodies (haploid) and one polar body (diploid) were carefully selected and were further cultured in NCSU 23 medium containing 0.4% bovine… CONTINUE READING