Handbook of Biological Confocal Microscopy

@inproceedings{Pawley1990HandbookOB,
  title={Handbook of Biological Confocal Microscopy},
  author={James B. Pawley},
  booktitle={Springer US},
  year={1990}
}
  • J. Pawley
  • Published in Springer US 1990
  • Physics, Biology
Foundations of Confocal Scanned Imaging in Light Microscopy -- Fundamental Limits in Confocal Microscopy -- Special Optical Elements -- Points, Pixels, and Gray Levels: Digitizing Image Data -- Laser Sources for Confocal Microscopy -- Non-Laser Light Sources for Three-Dimensional Microscopy -- Objective Lenses for Confocal Microscopy -- The Contrast Formation in Optical Microscopy -- The Intermediate Optical System of Laser-Scanning Confocal Microscopes -- Disk-Scanning Confocal Microscopy… 

Video-rate scanning confocal microscopy and microendoscopy.

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Confocal Laser-scanning Microscopy in Filamentous Fungi

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Defining the Tools: an Analysis of Laser Scanning Confocal and Wide-Field/Restoration Fluorescence Microscope Imaging

Two fundamentally different imaging technologies are available for imaging fluorescently labelled cells and tissues, in either the fixed or living state, and represent the majority of the fluorescence imaging systems currently in use in biomedical research.

Characterization and Use of Wide-Field Fluorescence Microscopy and Image Restoration in Quantitative Live Cell Imaging

Two fundamentally different imaging technologies are available for imaging fluorescently labelled cells and tissues, in either the fixed or living state, and represent the majority of the fluorescence imaging systems currently in use in biomedical research.

BLIND DECONVOLUTION IN 3D BIOLOGICAL MICROSCOPY

  • Physics
  • 2004
Confocal microscopy o ers several advantages over conventional optical microscopy with its small depth-ofeld, its reduction of out-of-focus blur, and its full three-dimensional (3D) image scanning

LASER SCANNING CONFOCAL MICROSCOPY

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High brightness LED in confocal microscopy

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Quantitative Fluorescence Confocal Laser Scanning Microscopy (CLSM)

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Laser scanning confocal microscopy: history, applications, and related optical sectioning techniques.

Significant improvements have been made to all areas of the confocal approach, not only to the instruments themselves, but also to the protocols of specimen preparation, to the analysis, the display, the reproduction, sharing and management of confocal images using bioinformatics techniques.
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References

SHOWING 1-10 OF 207 REFERENCES

Optical fluorescence microscopy in three dimensions: microtomoscopy

A unique property of confocal fluorescence microscopy is utilized to obtain scanned images of a number of samples in three dimensions. This kind of microscopy for the first time enables the direct

A standard video-rate confocal laser-scanning reflection and fluorescence microscope

A confocal laser‐scanning microscope (CLSM) differs from a conventional microscope by affording an extreme depth discrimination, as well as a slightly improved resolution. These features afford

Nondestructive Sectioning Of Fixed And Living Specimens Using A Confocal Scanning Laser Fluorescence Microscope: Microtomoscopy

The CSCM has to find its niche in the laboratories and this paper will present two applications in which it proves its usefulness.

Three-dimensional microscopy using a confocal laser scanning microscope.

In a scanning laser microscope detecting fluorescent light from the specimen, the depth-discriminating property of confocal scanning has been used to carry out optical slicing of a thick specimen.

3‐D image formation in high‐aperture fluorescence confocal microscopy: a numerical analysis

The imaging properties of a confocal fluorescence microscope are considered on the basis of a theoretical model. The model takes into account high‐aperture objectives, the polarization state of the

Optical sectioning in confocal fluorescent microscopes

We discuss the three‐dimensional imaging properties of confocal fluorescence scanning optical microscopes with particular reference to their optical sectioning property. We consider the practically

Potential clinical uses of laser scan microscopy.

Preliminary data indicate that the higher light sensitivity of CLSM allows detection of low amounts of proto-oncogene mRNA which are minimally detectable by LM, which could lead to more refined therapeutic indications.

The tandem scanning reflected light microscope

Reflected-light microscopy of semitransparent material, such as unstained nervous tissue, is usually unsatisfactory because of low contrast and light scattering. In a new microscope both the object

Observation of the rabbit cornea and lens with a new real‐time confocal scanning optical microscope

A new one-sided tandem scanning microscope has been utilized to optically section a transparent cornea and ocular lens with submicron depth and transverse resolution, which provides a new paradigm for cell biology and diagnostic ophthalmology.
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