Handbook of Biological Confocal Microscopy

  title={Handbook of Biological Confocal Microscopy},
  author={James B. Pawley},
  booktitle={Springer US},
  • J. Pawley
  • Published in Springer US 1990
  • Materials Science
Foundations of Confocal Scanned Imaging in Light Microscopy -- Fundamental Limits in Confocal Microscopy -- Special Optical Elements -- Points, Pixels, and Gray Levels: Digitizing Image Data -- Laser Sources for Confocal Microscopy -- Non-Laser Light Sources for Three-Dimensional Microscopy -- Objective Lenses for Confocal Microscopy -- The Contrast Formation in Optical Microscopy -- The Intermediate Optical System of Laser-Scanning Confocal Microscopes -- Disk-Scanning Confocal Microscopy… Expand
Video-rate scanning confocal microscopy and microendoscopy.
A step-by-step method is provided for the complete construction of a custom, video-rate confocal imaging system from basic components, enabling rapid, high-sensitivity, and high-resolution optical sectioning of complex systems. Expand
Deconvolution and Denoising for Confocal Microscopy
Fluorescence light microscopes, especially the confocal laser scanning microscopes, have become a powerful tool in life sciences for observing biological samples in order to detect the distributionExpand
Confocal Laser-scanning Microscopy in Filamentous Fungi
Confocal microscopy is an advanced fluorescence microscopy technique that utilizes laser illumination, optical devices, pinholes, computers, and cameras/detectors to perform cell and subcellularExpand
Defining the Tools: an Analysis of Laser Scanning Confocal and Wide-Field/Restoration Fluorescence Microscope Imaging
Digital fluorescence microscopy is now a standard tool for determining the localization of cellular components in fixed and living cells. Two fundamentally different imaging technologies areExpand
Characterization and Use of Wide-Field Fluorescence Microscopy and Image Restoration in Quantitative Live Cell Imaging
Digital fluorescence microscopy is now a standard tool for determining the localization of cellular components in fixed and living cells. Two fundamentally different imaging technologies areExpand
  • 2004
Confocal microscopy o ers several advantages over conventional optical microscopy with its small depth-ofeld, its reduction of out-of-focus blur, and its full three-dimensional (3D) image scanningExpand
Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living and fixedExpand
High brightness LED in confocal microscopy
We have introduced a novel illumination system for line scanning confocal microscopy. Confocal microscopy is a popular imaging tool in many applications specifically in medical imaging. Line scanningExpand
Quantitative Fluorescence Confocal Laser Scanning Microscopy (CLSM)
The confocal imaging geometry provides a dramatic optical advantage for fluorescence microscopy by discriminating against out-of-focus background with minimal loss of image-forming signal.Expand
Laser scanning confocal microscopy: history, applications, and related optical sectioning techniques.
Significant improvements have been made to all areas of the confocal approach, not only to the instruments themselves, but also to the protocols of specimen preparation, to the analysis, the display, the reproduction, sharing and management of confocal images using bioinformatics techniques. Expand


Optical fluorescence microscopy in three dimensions: microtomoscopy
A unique property of confocal fluorescence microscopy is utilized to obtain scanned images of a number of samples in three dimensions. This kind of microscopy for the first time enables the directExpand
An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy
It is found that confocal imaging gives greatly enhanced images of biological structures viewed with epifluorescence, and the improvements are such that it is possible to optically section thick specimens with little degradation in the image quality of interior sections. Expand
A standard video-rate confocal laser-scanning reflection and fluorescence microscope
A confocal laser‐scanning microscope (CLSM) differs from a conventional microscope by affording an extreme depth discrimination, as well as a slightly improved resolution. These features affordExpand
Nondestructive Sectioning Of Fixed And Living Specimens Using A Confocal Scanning Laser Fluorescence Microscope: Microtomoscopy
Modern molecular biologists and in particular cell biologists have a large set of experimental tools at their disposal. Immunocytochemistry, fluorescence labels, and microscopy are only subsets ofExpand
Three-dimensional microscopy using a confocal laser scanning microscope.
In a scanning laser microscope detecting fluorescent light from the specimen, the depth-discriminating property of confocal scanning has been used to carry out optical slicing of a thick specimen.Expand
Tandem scanning reflected-light microscopy of cell-substratum adhesions and stress fibres in Swiss 3T3 cells.
  • S. Paddock
  • Biology, Medicine
  • Journal of cell science
  • 1989
The TSM is used to produce improved images of cellular structures in 3T3 cells stained with various protein dyes including Napthol Blue Black (NBB) and Coomassie Brilliant Blue (CBB), and features of the nucleus, the cytoskeleton and extracellular matrix are resolved in both NBB- and CBB-stained cells. Expand
3‐D image formation in high‐aperture fluorescence confocal microscopy: a numerical analysis
The imaging properties of a confocal fluorescence microscope are considered on the basis of a theoretical model. The model takes into account high‐aperture objectives, the polarization state of theExpand
Three-dimensional reconstruction of neurons in the lamprey spinal cord in whole-mount, using a confocal laser scanning microscope
A technique by which 'optical sectioning' and three-dimensional reconstruction of fluorescence-labelled neurons in the lamprey spinal cord has been performed by means of a confocal microscope scanner with a laser beam as the light source. Expand
Resonance energy transfer microscopy: visual colocalization of fluorescent lipid probes in liposomes.
Publisher Summary This chapter outlines the modifications to a standard epifluorescence microscope for resonance energy transfer (RET) microscopy of one particular donor/acceptor pair, andExpand
Optical sectioning in confocal fluorescent microscopes
We discuss the three‐dimensional imaging properties of confocal fluorescence scanning optical microscopes with particular reference to their optical sectioning property. We consider the practicallyExpand