Handbook of Biological Confocal Microscopy

  title={Handbook of Biological Confocal Microscopy},
  author={James B. Pawley},
  booktitle={Springer US},
  • J. Pawley
  • Published in Springer US 1990
  • Physics, Biology
Foundations of Confocal Scanned Imaging in Light Microscopy -- Fundamental Limits in Confocal Microscopy -- Special Optical Elements -- Points, Pixels, and Gray Levels: Digitizing Image Data -- Laser Sources for Confocal Microscopy -- Non-Laser Light Sources for Three-Dimensional Microscopy -- Objective Lenses for Confocal Microscopy -- The Contrast Formation in Optical Microscopy -- The Intermediate Optical System of Laser-Scanning Confocal Microscopes -- Disk-Scanning Confocal Microscopy… 

Video-rate scanning confocal microscopy and microendoscopy.

A step-by-step method is provided for the complete construction of a custom, video-rate confocal imaging system from basic components, enabling rapid, high-sensitivity, and high-resolution optical sectioning of complex systems.

Confocal Laser-scanning Microscopy in Filamentous Fungi

This chapter focuses primarily on confocal microscopy used to image fungal cells, an advanced fluorescence microscopy technique that utilizes laser illumination, optical devices, pinholes, computers, and cameras/detectors to perform cell and subcellular visualization.

Deconvolution and Denoising for Confocal Microscopy

The aim of this chapter is to introduce the reader to the basics of the light and confocal microscopes, their imaging limitations, and the mathematics involved in the resolution and signal-to-noise ratio improvement methodologies.

Defining the Tools: an Analysis of Laser Scanning Confocal and Wide-Field/Restoration Fluorescence Microscope Imaging

Two fundamentally different imaging technologies are available for imaging fluorescently labelled cells and tissues, in either the fixed or living state, and represent the majority of the fluorescence imaging systems currently in use in biomedical research.

Characterization and Use of Wide-Field Fluorescence Microscopy and Image Restoration in Quantitative Live Cell Imaging

Two fundamentally different imaging technologies are available for imaging fluorescently labelled cells and tissues, in either the fixed or living state, and represent the majority of the fluorescence imaging systems currently in use in biomedical research.


  • Physics
  • 2004
Confocal microscopy o ers several advantages over conventional optical microscopy with its small depth-ofeld, its reduction of out-of-focus blur, and its full three-dimensional (3D) image scanning


Laser scanning confocal microscopy has become an invaluable tool for a wide range of investigations in the biological and medical sciences for imaging thin optical sections in living and fixed

High brightness LED in confocal microscopy

We have introduced a novel illumination system for line scanning confocal microscopy. Confocal microscopy is a popular imaging tool in many applications specifically in medical imaging. Line scanning

Quantitative Fluorescence Confocal Laser Scanning Microscopy (CLSM)

The confocal imaging geometry provides a dramatic optical advantage for fluorescence microscopy by discriminating against out-of-focus background with minimal loss of image-forming signal.

Laser scanning confocal microscopy: history, applications, and related optical sectioning techniques.

Significant improvements have been made to all areas of the confocal approach, not only to the instruments themselves, but also to the protocols of specimen preparation, to the analysis, the display, the reproduction, sharing and management of confocal images using bioinformatics techniques.



Optical fluorescence microscopy in three dimensions: microtomoscopy

A unique property of confocal fluorescence microscopy is utilized to obtain scanned images of a number of samples in three dimensions. This kind of microscopy for the first time enables the direct

A standard video-rate confocal laser-scanning reflection and fluorescence microscope

A confocal laser‐scanning microscope (CLSM) differs from a conventional microscope by affording an extreme depth discrimination, as well as a slightly improved resolution. These features afford

Nondestructive Sectioning Of Fixed And Living Specimens Using A Confocal Scanning Laser Fluorescence Microscope: Microtomoscopy

The CSCM has to find its niche in the laboratories and this paper will present two applications in which it proves its usefulness.

Three-dimensional microscopy using a confocal laser scanning microscope.

In a scanning laser microscope detecting fluorescent light from the specimen, the depth-discriminating property of confocal scanning has been used to carry out optical slicing of a thick specimen.

3‐D image formation in high‐aperture fluorescence confocal microscopy: a numerical analysis

The imaging properties of a confocal fluorescence microscope are considered on the basis of a theoretical model. The model takes into account high‐aperture objectives, the polarization state of the

Optical sectioning in confocal fluorescent microscopes

We discuss the three‐dimensional imaging properties of confocal fluorescence scanning optical microscopes with particular reference to their optical sectioning property. We consider the practically

Potential clinical uses of laser scan microscopy.

Preliminary data indicate that the higher light sensitivity of CLSM allows detection of low amounts of proto-oncogene mRNA which are minimally detectable by LM, which could lead to more refined therapeutic indications.

The tandem scanning reflected light microscope

Reflected-light microscopy of semitransparent material, such as unstained nervous tissue, is usually unsatisfactory because of low contrast and light scattering. In a new microscope both the object

Observation of the rabbit cornea and lens with a new real‐time confocal scanning optical microscope

A new one-sided tandem scanning microscope has been utilized to optically section a transparent cornea and ocular lens with submicron depth and transverse resolution, which provides a new paradigm for cell biology and diagnostic ophthalmology.