Hand-made cloned buffalo (Bubalus bubalis) embryos: comparison of different media and culture systems.

Abstract

Hand-made cloning (HMC) has proved to be an efficient alternative to the conventional micromanipulator-based technique in some domestic animal species. This study reports the development of an effective culture system for in vitro culture of zona-free cloned buffalo (Bubalus bubalis) embryos reconstructed using adult skin fibroblast cells as nucleus donor… (More)
DOI: 10.1089/clo.2008.0033

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