Hairy root culture of Artemisia annua L. by Ri plasmid transformation and biosynthesis of artemisinin.

The hairy root culture system of the medical plant Artemisia annua L. was established by infection with Agrobacterium rhizogenes R1601. The transgenic state of transformed roots was confirmed by Southern blot hybridization with TL-DNA of pFw302. The expression of NPTII gene was confirmed by enzymic assay. The important secondary metabolites-artemisinin was… CONTINUE READING