Hairpin-loop formation by inverted repeats in supercoiled DNA is a local and transmissible property.

@article{Lilley1981HairpinloopFB,
  title={Hairpin-loop formation by inverted repeats in supercoiled DNA is a local and transmissible property.},
  author={David M. J. Lilley},
  journal={Nucleic acids research},
  year={1981},
  volume={9 6},
  pages={1271-89}
}
Short inverted repeat sequences adopt hairpin stem-loop type structures in supercoiled closed circular DNA molecules, demonstrated by S1 nuclease cleavage. Fine mapping of cleavage frequencies is in good agreement with expected cleavage patterns based upon the interaction between an unpaired loop and a sterically bulky enzyme molecule. Whilst the topological properties of underwound DNA circles depend ultimately upon reduced linkage, necessarily a global molecular property, hairpin loop… CONTINUE READING