Haemopoiesis in human fetal and embryonic liver

  title={Haemopoiesis in human fetal and embryonic liver},
  author={Wim Timens and Willem A. Kamps and Thea Rozeboom-Uiterwijk and Sibrand Poppema Frcp},
  journal={Virchows Archiv A},
Paraffin embedded tissue reactive monoclonal antibodies were used to study human embryonal and fetal haemopoiesis, combining optimal morphology with immunohistological determination of haemopoietic cell subtypes and their microenvironment. Seven embryonal and twelve fetal liver specimens were studied, having been fixed in B5-fixative and embedded in paraffin. The different haemopoietic lineages each showed their own immunophenotype and distribution; intercellular and microenvironmental… 
Histochemical in situ identification of bovine embryonic blood cells reveals differences to the adult haematopoietic system and suggests a close relationship between haematopoietic stem cells and primordial germ cells
Cryostat sections of bovine embryos of exactly known age were treated with a wide range of antibodies directed against cell surface antigens or lineage-specific factors in order to demonstrate different types of fetal blood cells and their precursors.
Immunocytochemical observations on macrophage populations in normal fetal and adult human liver
It is concluded that the population of human liver macrophages is heterogeneous from an early stage of fetal development and that this heterogeneity extends into adult life.


Differential tissue distribution and ontogeny of DC-1 and HLA-DR antigens
In fetuses up to 26 weeks of age, DC-1 antigens were detected only on cortical and medullary thymic dendritic cells with an anatomic distribution similar to that of reticuloepithelial cells and in endothelial cells of the small intestine, whereas at this stage of intrauterine life, HLA-DR antIGens have already reached their full tissue distribution.
Immuno-architecture of human fetal lymphoid tissues
The use of paraffin tissue reactive (monoclonal) antibodies gives an extra dimension to the study of fetal lymphoid tissues, of particular advantage in studies on very fragile tissues as in early embryonal and fetal ontogeny.
Use of monoclonal antibodies in formol-paraffin sections
Thirteen monoclonal antibodies reactive with B- and T-lymphocytes, monocytes, granulocytes and erythrocytes were tested in sections fixed with formalin, formalin-sublimate or formal in-acetic acid and most of the antigens were not detectable.
Immunohistochemistry in Bone Marrow Diagnosis: Value of a Panel of Monoclonal Antibodies on Routinely Processed Bone Marrow Biopsies
A small panel of selected antibodies, such as UEA, Leu M-1, and LCA, and the immunoglobulin light chain antisera can be very helpful in bone marrow diagnosis and would cover most indications.
In Situ Study of Haemopoiesis in Human Fetal Liver
The expression of CD7 on haemopoietic fetal liver cells suggests that T‐cell precursors develop in fetal liver as well as B cells, and further delineation of these lineages is needed.
The developing human
The developing human : , The developing human : , کتابخانه دیجیتالی دانشگاه علوم پزشکی و خدمات درمانی شهید بهشتی
Characterization of adult human marrow hematopoietic progenitors highly enriched by two-color cell sorting with My10 and major histocompatibility class II monoclonal antibodies.
Monoclonal antibodies, My10 (HPCA-1) and major histocompatibility class II (HLA-DR), were used to enrich and phenotype normal human marrow colony-forming unit and assess responsiveness to the effects of recombinant human tumor necrosis factor-alpha, recombinanthuman interferon-gamma, and prostaglandin E1.
Monoclonal Antibodies in Lymphoma Diagnosis and Classification.
The availability of monoclonal antibodies reactive with antigens present on leukocytes and leukocyte subpopulations has enabled a more reliable diagnosis and a more precise subclassification of
Monoclonal antibodies (MT1, MT2, MB1, MB2, MB3) reactive with leukocyte subsets in paraffin-embedded tissue sections.
Five new antibodies reactive with leukocyte subsets in formaldehyde-fixed, paraffin-embedded tissue sections in lymphomas indicate that the MB and MT antibodies are useful tools in the study of reactive and neoplastic disorders of the lymphoid system.
Human embryonic hemopoiesis. Kinetics of progenitors and precursors underlying the yolk sac----liver transition.
The results provide circumstantial support to a monoclonal hypothesis for human embryonic hemopoiesis, based on migration of stem and early progenitor cells from a generation site (YS) to a colonization site (L) via circulating blood.