Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris

@article{Bankefa2018Hac1pHF,
  title={Hac1p homologues from higher eukaryotes can improve the secretion of heterologous proteins in the yeast Pichia pastoris},
  author={Olufemi Emmanuel Bankefa and Meiyu Wang and Taicheng Zhu and Yin Li},
  journal={Biotechnology Letters},
  year={2018},
  volume={40},
  pages={1149-1156}
}
ObjectivesTo systematically explore the effects of overexpressing Hac1p homologues from different sources on protein secretion in Pichia pastoris system.ResultsEffects of Hac1p homologues encompassing P. pastoris (PpHac1p), S. cerevisiae (ScHac1p), Trichoderma reesei (TrHac1p) and Homo sapiens (HsXbp1), on secretion of three reporter proteins—β-galactosidase, β-mannanase and glucose oxidase were investigated. No individual Hac1p was optimal for all the enzymes. Rather, by testing a set of Hac1p… Expand
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Overexpression of P. pastoris Hac1p can be used to increase the production of heterologous proteins but needs to be evaluated on a case by case basis. Expand
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