HVJ-envelope vector for gene transfer into central nervous system.

  title={HVJ-envelope vector for gene transfer into central nervous system.},
  author={Munehisa Shimamura and Ryuichi Morishita and Masayuki Endoh and Kazuo Oshima and Motokuni Aoki and Satoshi Waguri and Yasuo Uchiyama and Yasufumi Kaneda},
  journal={Biochemical and biophysical research communications},
  volume={300 2},
To overcome some problems of virus vectors, we developed a novel non-viral vector system, the HVJ-envelope vector (HVJ-E). In this study, we investigated the feasibility of gene transfer into the CNS using the HVJ-E both in vitro and in vivo. Using the Venus reporter gene, fluorescence could be detected in cultured rat cerebral cortex neurons and glial cells. In vivo, the reporter gene (Venus) was successfully transfected into the rat brain by direct injection into the thalamus… CONTINUE READING