HSV-1 amplicon-mediated transfer of 128-kb BMP-2 genomic locus stimulates osteoblast differentiation in vitro.

@article{Xing2004HSV1AT,
  title={HSV-1 amplicon-mediated transfer of 128-kb BMP-2 genomic locus stimulates osteoblast differentiation in vitro.},
  author={Weirong Xing and David J Prof Baylink and Chandrasekhar Kesavan and Subburaman Mohan},
  journal={Biochemical and biophysical research communications},
  year={2004},
  volume={319 3},
  pages={
          781-6
        }
}
In previous studies, we developed mouse genetic models and discovered genetic components of quantitative trait loci on mouse chromosomes that contribute to phenotypes such as bone size, bone density, and fracture healing. However, these regions contain dozens of genes in several overlapping bacterial artificial chromosomes (BACs) and are difficult to clone by physical cloning strategies. A feasible and efficient approach of identifying candidate genes is to transfer the genomic loci in BAC… CONTINUE READING