HPLC with enzymatic detection as a candidate reference method for serum creatinine.

@article{Linnet1991HPLCWE,
  title={HPLC with enzymatic detection as a candidate reference method for serum creatinine.},
  author={Kristian Linnet and Ivan Bruunshuus},
  journal={Clinical chemistry},
  year={1991},
  volume={37 10 Pt 1},
  pages={1669-75}
}
We present a candidate Reference Method for determining the concentration of serum creatinine. The method is based on HPLC combined with enzymatic determination. Creatinine plus 14[C]creatinine is extracted by cation-exchange chromatography, subjected to reversed-phase HPLC, and finally quantified enzymatically. Enzymatic measurement ensures no interference from co-eluting compounds, which has been a problem for some reported HPLC assays relying on ultraviolet detection. The average corrected… CONTINUE READING

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Determination of creatinune in serum with HPLC and column switching

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