HPLC studies on leukotriene A4 obtained from the hydrolysis of its methyl ester.

@article{Carrier1988HPLCSO,
  title={HPLC studies on leukotriene A4 obtained from the hydrolysis of its methyl ester.},
  author={D J Carrier and T Bogri and Gregory Cosentino and Ingrid Guse and Suman Rakhit and Karan P. Singh},
  journal={Prostaglandins, leukotrienes, and essential fatty acids},
  year={1988},
  volume={34 1},
  pages={27-30}
}
Alkaline hydrolysis of leukotriene A4 methyl ester to leukotriene A4 was studied in either methanol or acetone. Hydrolysis in acetone yielded larger amounts of leukotriene A4 than similar hydrolysis in methanol. The maximum amount was obtained 60 minutes after the beginning of the hydrolysis. Leukotriene A4, as well as leukotriene B4 methoxy isomers were obtained from hydrolysis of leukotriene A4 methyl ester in methanol. It was found that initial leukotriene A4 methyl ester concentration… CONTINUE READING