HPLC separation of DL-amino acids derivatized withN2-(5-fluoro-2,4-dinitrophenyl)-L-amino acid amides

@article{Brckner1990HPLCSO,
  title={HPLC separation of DL-amino acids derivatized withN2-(5-fluoro-2,4-dinitrophenyl)-L-amino acid amides},
  author={H. Br{\"u}ckner and C. Keller-Hoehl},
  journal={Chromatographia},
  year={1990},
  volume={30},
  pages={621-629}
}
SummaryNeutral, acidic, and basic protein DL-amino acids (DL-AA) have been separated by HPLC as diastereomeric derivatives obtained after derivatization withN2-(5-fluoro-2,4-dinitrophenyl)-L-alanine amide (FDNP-Ala-NH2, “Marfey's reagent”). HPLC was performed on 3 μm Spherisorb ODS II as the stationary phase with gradient elution using mixtures of triethylammonium phosphate buffer (pH 3) and acetonitrile. The differences in retention times (ΔtR) of diastereomers were compared with those… Expand
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