• Corpus ID: 74727747

HPLC determination of wedelolactone in a market herbal extract sample and its validation

@inproceedings{Yuliana2011HPLCDO,
  title={HPLC determination of wedelolactone in a market herbal extract sample and its validation},
  author={Trisna Yuliana and Muljadji Agma and Iwan Hastiawan and Julia Kantasubrata},
  year={2011}
}

• T. Yuliana, M. Agma, +1 author J. Kantasubrata
• Published 2011
• Mathematics

Wedelolactone has a wide range of biological activities and used for the treatment various ailments. In the present study an attempt has been developed and validation for the quantification of wedelolactone. Analysis of wedelolactone was performed by HPLC using linear gradient and applied to the herbal extract samples from the local market. The linear regression data analysis for the calibration plots showed good linear relationship with r = 0.9961 in the concentration range 11.0 – 90.9 ug.mL -1 . The present method was validated for precision, recovery, limit of detection and limit of quantitation. Statistical analysis of the data showed that the method is reproducible and selective for the estimation of wedelolactone.