HPLC determination of thiopurine nucleosides and nucleotides in vivo in lymphoblasts following mercaptopurine therapy.

@article{Dervieux2002HPLCDO,
  title={HPLC determination of thiopurine nucleosides and nucleotides in vivo in lymphoblasts following mercaptopurine therapy.},
  author={Thierry Dervieux and Yaqin Chu and Yi Su and Ching-Hon Pui and William E. Evans and Mary V. Relling},
  journal={Clinical chemistry},
  year={2002},
  volume={48 1},
  pages={61-8}
}
BACKGROUND Mercaptopurine is a prodrug requiring intracellular activation to thiopurine nucleotides to exert antileukemic effect. We developed a reversed-phase liquid chromatographic assay for the quantification of mercaptopurine, thioguanine, and methylmercaptopurine nucleoside and nucleotide concentrations in the target tissue, the leukemic lymphoblast. METHODS Leukemic blasts were isolated from peripheral blood and bone marrow by a standard Ficoll-hypaque procedure. Proteins were removed… CONTINUE READING

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Possible implication of thiopurine S-methyltransferase in the occurrence of infectious episodes during maintenance therapy of acute leukemia with mercaptopurine

  • T Dervieux, Y Medard, P Verpillat, V Guigonis, M Duval, B Lescoeur
  • Leukemia
  • 2001
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